Fig. 5

miR-504 regulates Wnt–β-catenin signaling activation. a TOP/FOPFlash reporter activity assay detection of relative Wnt signaling activity in U87 and U373 cells. b Western blot assessment of the effects of miR-504 on β-catenin, p-GSK3β, GSK3β, and c-MYC protein levels in U87 and U373 cells. c, d Western blot (c) and immunofluorescence (d) assay detection of nuclear accumulation of β-catenin in U87 cells. e, f qRT-PCR analysis of the relative mRNA expression of downstream target genes of the Wnt–β-catenin pathway, i.e., CD44 (e) and c-MYC (f), in U87 and U373 cells. Data are the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001