Fig. 6

miR-504 inhibits GBM cell ME transition by suppressing the FZD7-mediated Wnt–β-catenin pathway. a, b qRT-PCR (a) and western blot (b) of FZD7 expression in miR-504–transfected U87 and U373 cells after FZD7 transfection. c, d Wound healing assay (c) and Transwell assay (d) evaluation of the restorative effect of FZD7 on miR-504–induced inhibition of migration and invasion in U87 and U373 cells (scale bar = 100 μm). e Western blot detection of EMT markers in U87 and U373 cells co-transfected with miR-504 and FZD7. f TOP/FOPFlash reporter activity detection of relative Wnt signaling activity in U87 and U373 cells. g Western blot detection of β-catenin, p-GSK3β, and GSK3β expression levels in miR-504 and FZD7 co-transfected cells. h Western blot assessment of the effects of FZD7 on nuclear β-catenin, c-MYC, and CD44 protein levels in U87 cells. i The diagram illustrated that miR-504 decreased the EMT and stemness via inhibiting the FZD7 mediated Wnt/β-catenin signaling pathway activity. Data are the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001