Fig. 1
Prss14/ST14 is critical for metastasis of orthotopically-implanted 4 T1 and E0771 breast cancer cells. (a) Survival curves of host mice orthotopically injected with 4 T1 and 4 T1EpiKD cells. 1 × 102, 1 × 103, 1 × 104, 1 × 105, or 1 × 106 of 4 T1 control (Con; solid lines) or 4 T1EpiKD (EpiKD; dotted lines) cells were implanted into the mammary fat pad of Balb/c mice. The cell numbers are indicated in the graph. (b) The growth curves of 4 T1Con and 4 T1EpiKD primary tumors. From top to bottom, 1 × 104, 1 × 105, and 1 × 106 cells. (c) The weight of 4 T1 primary tumor and the numbers of 4 T1 lung metastatic nodules. Upper panels: primary tumor weight, lower panels: nodule numbers. Filled circle: 4 T1Con, empty circle: 4 T1EpiKD. Means and standard errors are indicated. (d) Survival rate of C57BL/6 mice implanted with E0771-Control-D11 (E0771Con, solid line) or E0771-EpiKD-C3 (E0771EpiKD, dotted line) cells (1 × 104, 1 × 105, 1 × 106 cells/mouse (n = 10)). (e) The growth curves of E0771Con and E0771EpiKD primary tumors. (f) Comparison of the E0771 primary tumor weight and E0771 lung weight. Filled circle: E0771Con, empty circle: E0771EpiKD. P values measured by unpaired t test are shown