Fig. 6
mAb3F3 can reduce cell migration and metastasis of MMTV-PyMT breast cancer. (a) Wound healing migration assay with 4 T1. mAb3F3 culture supernatant was added to the final concentration of 10%. (b) Transwell migration assay with MCF7. Dose response curves and representative fields are at the bottom. mAb5: Solid line, mAb3F3: Dotted line. (c) 5 × 104 cells of MCF7 were seeded in 12 well plates. After 12 h, MCF7 were treated with mAb3F3 or control. Number of cells were checked every 24 h. (d) Cell cycle analysis by Propidium Iodide staining. Same numbers of MCF7 cells were seeded. (e) Growth curves of average tumor sizes from PBS, Taxol, and mAb3F3 injected PyMT mice. Arrows indicate injection time points (days) of mAb3F3. (f) Tumor volumes of each group at day11. (g) Number of metastasis nodules at day14. (h) Representative lung images of each group at the bottom