Fig. 1
From: P53-R273H mutation enhances colorectal cancer stemness through regulating specific lncRNAs
P53-R273H as to p53-R175H and p53-R248W exhibits more invasive ability and promote CSC expansion. a. Transwell assay of HCT116 p53−/− cells overexpression of R175H, R248W and R273H mutants. Representative images (left) and statistics (right) of migration and invasion assay were shown. OE, overexpression. b. The spheroids size (left) and number (right) of 1st spheroid formation (left, upper) and 2nd spheroid formation (left, down) assay of over-expression cell lines. Scale bar, 400 μM. c. Relative expression of stemness-related genes (Sox2, Oct4, Nanog) in over-expression cell lines was quantified by RT-qPCR. β-Actin mRNA was used as an internal control. The experiments were performed in triplicate. Data are shown as means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001