Fig. 1

Identification of miR-3064 as a potential oncogenic miRNA in PC. (a) Heatmap of miRNAs expression in human PC tissues (n = 5) compared with normal tissues (n = 5) measured by miRNA microarray. (b) qRT-PCR analysis of indicated miRNAs in PC tissues (n = 5) and normal tissues (n = 5). (c) Effects of transfection with 11 upregulated miRNAs on PC cell proliferation. (D) Expression of miR-3064 in PC tissues and normal tissues using qRT-PCR analysis (n = 50). (e) Levels of miR-3064 in PCs with lymph node metastasis tissues and PCs without node metastasis. (f) The expression of miR-3064 was examined in five PC cell lines and a normal pancreatic cell line HPDE6-C7 using qRT-PCR analysis. (g) Kaplan-Meier curves for overall survival of PC patients with low or high miR-3064 levels. (h) Kaplan–Meier analysis for overall survival based on low or high miR-3064 levels (the KMPlotter database). *P < 0.05