Fig. 5

ACLY is positively correlated with CTNNB1, both correlated with tumor metastasis and patient survival. a Representative images of staining with ACLY and CTNNB1 antibodies in colon cancer tissue showed strong, moderate, and weak expression, respectively (scale bar: 100 μm). b, c Statistical analysis of ACLY staining (b) and CTNNB1 staining (c) between colon cancer tissues and adjacent normal tissues or metastasis and no-metastasis tissues. d Pearson correlative analysis of staining scores for ACLY and CTNNB1. e Pearson correlative analysis of staining scores for ACLY and CTNNB1 in the metastasis subgroup. f Kaplan-Meier overall survival analysis of colon cancer patients. ACLY^highCTNNB1^high (red curve), ACLY^highCTNNB1^low (Blue curve, P = 0.0005), ACLY^lowCTNNB1^high (Black curve, P = 0.0674) and ACLY^lowCTNNB1^low (Green curve, P < 0.0001) group was showed (P value is obtained by Kaplan–Meier analysis between ACLY^highCTNNB1^high group and other groups)