Fig. 1

QW24 inhibited colorectal cancer cells proliferation and down-regulated BMI-1. a, Chemical structure of QW24 with the molecular weight (Mol. Wt.) of 444.5 g/mol. b, LS174T, HCT116, HT29, CT26, HCT8, HCT15 and LoVo cells were seeded in 96-well plates (3000 cells/well) and treated with 0, 0.5, 1, 2, 4 μM of QW24 after cells were attached. After 72 h incubation, cell growth was determined by SRB assay. c, The IC50 of QW24 for different colorectal cancer cell lines were listed. d, The IC50 of QW24 for normal cell lines were listed, including human normal liver cell L02, human skin fibroblast cell HAF, human normal colon epithelium cell NCM460 and human umbilical vein endothelial cell HUVEC. e, HCT116 cells were seeded in 96-well plates (3000 cells/well) and treated with 0, 0.5, 1, 2, 4 μM of QW24 or PTC-209 after cells were attached. After 72 h incubation, cell growth was determined by SRB assay. f, HCT116 cells were treated with 0, 1, 2, 4 μM of QW24 or PTC-209 for 12 h. Cells were lysed and BMI-1 protein level was measured by western blotting analysis. g, LS174T, HCT116, HT29, and CT26 cells were treated with 0, 2, 4 μM of QW24 for 12 h. Cells were lysed and BMI-1, Ub-H2A and H2A protein levels were measured by western blotting analysis. h and i, HCT116 cells were infected with increasing doses of empty vector lentivirus (control) or BMI-1 lentivirus for 72 h. Cells were seeded in 96-well plates (3000 cells/well) and treated with indicated concentrations of PTC-209 (h) or QW24 (i) after cells were attached. After 96 h incubation, cell growth was measured by SRB assay. Data are presented as mean ± s.d. (n = 5); **, P < 0.01; ***, P < 0.001