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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: A novel BMI-1 inhibitor QW24 for the treatment of stem-like colorectal cancer

Fig. 6

QW24 inhibited the hepatic metastases of colorectal tumor in vivo and extended the lifetime of mice. a, Male BALB/c-nude mice were anesthetized. CT26-luciferase colon cancer cells were surgically injected into the spleen. One week after injection, mice were administrated with splenectomy and mice were respectively i.p. injected with DMSO (n = 9), PTC-209 30 mg/kg (n = 9), QW24 30 mg/kg (n = 10) every day for 12 days. Hepatic metastases were monitored every 3 days using the IVIS Imaging System. Representative images of 3 mice per group were illustrated. Measurements of bioluminescent signals were acquired and analyzed using Living Image and Xenogen software (n ≥ 9). b, Mice body weight was measured every 2 days within treatment. c-e. After 12 days, mice were sacrificed and the livers of the mice were harvested, imaged to acquire bioluminescent signal and photographed to determine tumor cell dissemination. Representative images of hepatic metastases were showed, and the metastases were quantified and counted. Data are presented as mean ± s.d. (n ≥ 9); *, P < 0.05; **, P < 0.01; ***, P < 0.001. f, Animal model was generated as descripted in (a) and the mice were treated with DMSO (n = 10), PTC-209 30 mg/kg (n = 10) and QW24 30 mg/kg (n = 10) every day for 21 days. Then the survival rate of mice was recorded to 14 weeks and Kaplan-Meier survival curves were analyzed

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