Fig. 2

FAM289 is involved in GBM cells proliferation and migration in vitro and in vivo. (A) Real-time monitoring of FAM289-induced proliferation in U251 (a) or U87-MG (b) cells. Cell index was automatically recorded with the xCELLigence real-time cell analyzer (RTCA) every 15 min until the end of the experiment (60 h). Each tracing represents an average of three parallel assessments. (B) The effect of FAM289 on migrating capacity in U251 (a&b) and U87-MG (c&d) cells. The representative pictures were taken at 0 h, 24 h and 48 h after scratching. The differences were significant both at 24 h and at 48 h. (C) Transwell assay of FAM289-induced migrationin U251 (a&b) and U87-MG (c&d) cells. Images were obtained at 48 h. Data are presented as the mean ± SEM of three independent experiments. (D) Ectopic expression of FAM289 accelerated growth of FAM289 xenografts in NCG mice (n ≥ 5) as compared to controls. The representative tumor photos and summary volume & weight were presented in (a), (b) and (c) respectively. (E) Knockdown of FAM289 decelerated growth of U87-MG cell-derived xenografts in nude mice (n ≥ 5) as compared to controls (*p < 0.05 and **p < 0.01 vs. control). The representative tumor photos and summary volume & weight were presented in (a), (b) and (c) respectively. (F) The U251-Luc cells with/without overexpression FAM289 (2 × 10⁶ each) were subcutaneously injected into the brain of NCG mice (n ≥ 3) and the sizes of neoplasms formed were measured with an in vivo imaging system until mice died (usually 3~4 weeks). (a) Serial pictures taken at different time points; (b) The changes of luminescence radiance values of the neoplasms formed from the injected U251-Luc cells with/without overexpression FAM289. The difference was significant (*p < 0.05 and **p < 0.01 vs. control)