Fig. 3

HOTTIP stimulates IL-6 transcription via regulating c-jun. a The relationship between HOTTIP, IL-6 and c-jun predicted by LNCmap. b The binding relationship between HOTTIP and IL-6, as well as between c-jun and IL-6 confirmed by dual luciferase reporter gene assay. c The binding sites between c-jun and IL-6 promoter predicted and verified by ChIP assay. d The interaction between c-jun and HOTTIP validated by RIP assay. e Western blot analysis of IL-6 protein in SKOV3 cells overexpressing or silencing HOTTIP. f The secretion of IL-6 in SKOV3 cells overexpressing or silencing HOTTIP as measured by ELISA. ^* p < 0.05 vs. cells treated with IL-6-prom-wt plus NC; ^# p < 0.05 vs. cells without treatment; ^& p < 0.05 vs. cells treated with IgG; The measurement data were summarized as mean ± standard deviation. The unpaired t test was used to compare the data in two groups. The one-way analysis of variance was adopted to compare the data among multiple groups, followed by Tukey’s post hoc test. The experiment was repeated 3 times independently