Fig. 5

miR-200b/c levels are down-regulated in breast cancer under hypoxia and negatively regulated by circDENND4C. a-d miR-200b/c expression levels in breast cancer tissues (n = 43) and cells were measured by qRT-PCR. e-h The abundances of miR-200b and miR-200c were detected in MDA-MB-453 and SK-BR-3 cells after exposure of hypoxia. i-l The expression levels of miR-200b and miR-200c were measured in MDA-MB-453 and SK-BR-3 cells transfected with pcDNA, circDENND4C overexpression vector (circ), siRNA negative control (si-NC) or siRNA against circDENND4C (si-circ). *P < 0.05, **P < 0.01, ***P < 0.001