Fig. 1

HOXD9 is overexpressed in GC cell lines and tissues. a Whole lysates of GES-1, AGS, BGC-823, MGC-803, HGC-27, MKN-28, MKN-45 and SGC-7901 were collected, and HOXD9 was detected by Western blot. β-tubulin was used as the internal control. b The expression of HOXD9 by Western blot in 10 pairs of GC and matched noncancerous gastric tissues. N: normal; C: cancer. Arrow, tumor-associated stroma cells (TAS) (c) HOXD9 expression in normal and malignant human gastric tissues was detected by TMA. d Expression of HOXD9 was determined in normal and cancerous gastric tissues. ****, P < 0.001. e Representative IHC images are shown for HOXD9 expression in lymph node metastatic cancer tissues. f Expression of multiple transcription factor oncogenes in transient transfection as detected by qRT-PCR in GC cells. *, P > 0.05, **, P < 0.05, ***, P < 0.01. Scale bars, 100 μm in c & e