Fig. 5

HOXD9-RUFY3 axis promotes the growth and invasion of GC cells in vitro. a Expression levels of HOXD9 were detected by Western blot analysis in AGS cells, which were transfected with RUFY3 siRNA1, RUFY3 siRNA2 or scr siRNA (scrambled siRNA) as a negative control. b Soft agar colony formation assays using the indicated cell clones. c Stable transfectants of HOXD9 after transfection with RUFY3 siRNA1, RUFY3 siRNA2 or scr siRNA for 48 h were subjected to an EdU incorporation assay. d & e The GC cell migration and invasion assays were performed. f & g Representative gelatin zymography showing MMP2 and MMP9 activities in GC cells. g Quantitative analysis of (f). Bars represent the value of intensity of the bright band and are used for statistical analysis. N = 3, ****, P < 0.001 vector vs. HOXD9 and HOXD9 vs. HOXD9-RUFY3 siRNA, respectively. Scale bars, 100 μm in c