Fig. 3

miR-133a-3p promotes breast cancer cells proliferation and stemness in vitro. a-c Proliferation of MCF-7 cells (a), MDA-MB-468 cells (b) and MDA-MB-231 cells (c) transfected with mimics NC, miR-133a-3p mimics, inhibitor NC, or miR-133a-3p inhibitor detected by CCK8 assay. d-f The percentage of CD44+/CD24- population in MCF-7 cells (d), MDA-MB-468 cells (e), and MDA-MB-231 cells (f) transfected with mimics NC, miR-133a-3p mimics, inhibitor NC or miR-133a-3p inhibitor. g-i The protein levels of pluripotent transcription factors in MCF-7 cells (g), MDA-MB-468 cells (h), and MDA-MB-231 cells (i) transfected with mimics NC, miR-133a-3p mimics, inhibitor NC, or miR-133a-3p inhibitor by western blotting. *P < 0.05; **P < 0.01; ***P < 0.001