Fig. 6

Effects of MAML1-targeted miR-133a-3p on the lung and liver colonization of MDA-MB-231 cells xenografts in mice. a, b miR-133a-3p levels (a) and MAML1 protein levels (b) in MDA-MB-231 cells transfected with either the control lentivirus, miR-133a-3p lentivirus, MAML1 lentivirus, or miR-133a-3p lentivirus plus MAML1 lentivirus. c Experimental design: immunocompromised mice were injected through tail vein with MDA-MB-231 cells transfected with either the control lentivirus, miR-133a-3p lentivirus, MAML1 lentivirus, or miR-133a-3p lentivirus plus MAML1 lentivirus. d, e Representative BLI images (d) and quantitative analysis of the fluorescence intensities (e) of mice of four groups. The BLI was performed on days 10, 20, and 30 after injection. The intensity of BLI is represented by the color. f, g Representative H&E-stained sections of lung tissues (f) and liver tissues (g) isolated from the intravenously injected mice. Black arrows indicate metastatic nodules. Scale bar, 200/100 μm. h Representative immunohistochemical staining of MAML1 of lung tissues (upper) and liver tissues (lower) respectively. Scale bar, 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001