Fig. 5

METTL3 regulated the maturation of pri-miR-1246 by changing the m6A modification status of pri-miR-1246. (a) m6A modification was enriched in pri-miR-1246; The percentage of the input is shown. (b) The expression level of pri-miR-1246 and miR-1246 when METTL3 was knocked down in DLD-1 cell line. (c) m6A modification level in the presence of METTL3 depletion. (d) Right panel showed RRACU m6A motifs sites in the upstream of pri-miR-1246; Left panel showed the motif mutation. (e) Relative level of pri-miR-1246 upon transfected with wide-type pri-miR-1246 or mutant plasmids (left panel) in HEK293T cell line; Relative level of mature miR-1246 upon transfected with wide-type or mutant pri-miR-1246 plasmids (right panel) in HEK293T cell line. (f) Changes in m6A modification level of pri-miR-1246 between wide-type and mutant-type plasmids. Data are presented as means ± standard deviation (**P < 0.01, ***P < 0.001)