Fig. 3

CyclinD1 and H3K9me3 are collocated in the nucleus and form a complex with SUV39H1, HP1α and DNMTs in ICC cells. a Western blot analysis reveals that CyclinD1 and H3K9me3 are mainly expressed in the nucleus in HuccT-1 and HCCC9810 cells. b Immunofluorescence (IF) analysis of the nuclear co-localization of CyclinD1 with H3K9me3 in HuccT-1 and HCCC9810 cells (400×, magnification). c-f Anti-CyclinD1 immunoprecipitates endogenously with SUV39H1, H3K9me3 and HP1α from HuccT-1, HCCC9810 and HIBEpic cells