Fig. 5

The effect of CyclinD1 and/or Dicer silencing on the proliferation and invasion of ICC cells. HuccT-1 and HCCC9810 cells were transduced with Lv-NC (negative control), Lv-siR-CyclinD1 and/or Lv-siR-Dicer to establish stable silencing cells. The proliferation, invasion, wound healing and cell cycling were determined. The growth of implanted negative control, Lv-siR-CyclinD1 and Lv-siR-CyclinD1/lv-siR-dicer HuccT-1 tumors in BALB/c nude mice was monitored. a The dynamic proliferation of ICC cells. b The invasion of ICC cells (200×, magnification). c The wound healing of ICC cells. d The distribution of cell cycle status of ICC cells. e The growth of implanted HuccT-1 tumors in mice (N = 4 per group). Data are representative images or expressed as individual mea values or the means ± SD of each group from three separate experiments. *P < 0.05, **p < 0.01, ***p < 0.001