Fig. 5

Knockdown MEIS2C/D inhibited nuclear accumulation of YAP by miR-1307-3p/LATS1/YAP axis. a Diagrams indicate putative miR-1307-3p binding sites and corresponding mutant sites of LATS1 mRNA. LATS1 wild type mRNAs (WT) and corresponding mutant sequences (MUT), which was cloned in luciferase reporter and co-transfection of either negative control or miR-1307-3p mimic in HEK293T cells. b LATS1 protein and mRNA levels were measured by western blot and qRT-PCR in HEK293T cells transfected with miR-1307-3p mimic, anti-miR-1307-3p or negative control (NC) miRNA. c HCC-LM3 cells were transfected with anti-miR-1307-3p, miR-1307-3p mimic, MEIS2C/D siRNA or controls. Then, Immunoblotting of cell lysates was performed to examine phosphorylation of LATS1 and YAP. d HCC-LM3 cells were transfected with indicated siRNA, miRNA and inhibitor. YAP intracellular localization investigated by immunofluorescence staining