Fig. 3

YAP and JNK pathway are involved in WZ35 mediated breast cancer cell growth inhibition. a Western blot analysis of the Hippo pathway related proteins MST1, MST2, LATS1, MOB1, p-MOB1, p-YAP(s397), p-YAP(s127), YAP/TAZ and SAV1 in MDA-MB-231 cells treated with curcumin (10 μg/mL) or WZ35 (10 μg/mL). GAPDH was used as loading control. b Western blot analysis of p-JNK, AKT and p-AKT expression in MDA-MB-231 cells treated with WZ35 (10 μg/mL) or curcumin (10 μg/mL). c MDA-MB-231 cells were transfected with or without YAP siRNA and co-treated with WZ35 (10 μg/mL) for 12 h, the protein expression of YAP, JNK, p-JNK, BCL-2 and cleaved caspase-3 were determined by western blot. d MDA-MB-231 cells were transfected with or without YAP siRNA and co-treated with WZ35 (10 μg/mL) for 24 h, 48 h, 72 h respectively. Then cell viability was detected by CCK8 assay. e Representative images and the numbers of migrated cells were evaluated after treatment of MDA-MB-231 cells with Si-YAP, WZ35 and Si-YAP+WZ35. f Western blot analysis of YAP, JNK, p-JNK, BCL-2 and Cle-Caspase3 protein levels in MDA-MB-231 cells treated with YAP-OE, WZ35 and YAP-OE + WZ35. g Representative images and the numbers of migrated cells were evaluated after treatment of MDA-MB-231 cells with YAP-OE, WZ35 and YAP-OE + WZ35. h MDA-MB-231 cells were transfected with or without YAP-OE and co-treated with WZ35 (10 μg/mL) for 24 h, 48 h, 72 h respectively. The cell viability was detected by CCK8 assay. Data are presented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001