Fig. 4

WZ35 induces cytotoxicity in breast cancer cells is depending on ROS mediated YAP and JNK activation. a Flow cytometry analysis were performed to determine the intracellular ROS levels in MDA-MB-231 cells treated with curcumin or WZ35. b Intracellular ROS levels in MDA-MB-231 cells treated with WZ35 or WZ35 + NAC. c Cell proliferation ability was detected by CCK8 assay. d Representative images and the numbers of migration cells were evaluated after treatment of MDA-MB-231 cells with NAC, WZ35 and NAC + WZ35. e The expression of YAP, JNK and p-JNK in MDA-MB-231 cells treated with NAC, WZ35 and NAC + WZ35 were detected by western blot. f Cell proliferation ability was detected by CCK8 assay. g Representative images and the numbers of migrated cells were evaluated after treatment of MDA-MB-231 cells with SP600125, WZ35 and SP600125 + WZ35. h The expression of JNK and p-JNK proteins were determined by western blot. Data are presented as mean ± SD, **p < 0.01, ***p < 0.001