Fig. 5

FOXG1 activates Wnt/β-catenin signaling by inducing nuclear translocation of β-catenin. a Huh7 and PLC/PRF5y cells transfected with TOP Flash or FOP Flash, Renilla pRL-TK plasmids and ectopically expressed FOXG1 were subjected to dual-luciferase assays 48 h after transfection. Reporter activity was normalized to Renilla luciferase activity. The group of cells transfected with vector was used as controls. b qPCR detection of altered expression of Wnt signal-pathway genes after ectopic FOXG1 expression in the indicated cells. The group of cells transfected with vector was used as controls. c Altered nuclear levels of β-catenin and FOXG1 in response to down-regulated FOXG1 expression were detected by western blotting. P84 were used as a loading controls. d HCC cells were stained with fluorescent antibodies against β-catenin (red) or FOXG1 (green) and nuclei were counterstained with DAPI (blue). Representative confocal immunofluorescence images are shown