Fig. 7

FOXG1 is a TCF4 binding partner that enhances TCF4 activity. a Lysates of 293FT cells expressed myc-TCF4 and FLAG-FOXG1, or a control vector subjected to IP using anti–myc-TCF4 (Upper panel) and anti-FLAG-FOXG1 (lower panel) antibodies, followed by IB with anti-myc or anti-FLAG antibody. b Mutual dependence of FOXG1 and TCF4 in activating the TOP Flash reporter. FOP Flash was used as negative control. 293 T cells were cotransfected with FOXG1 expression plasmid, TCF4 siRNA, TCF dominant negative (TCF4 DN) expression plasmid, and TCF4-siRNA alone or in combination as indicated. Values are mean ± SD of triplicate samples. c ChIP assays of Wnt responsive enhancers (WREs) in the LEF-1 promoter or the ORF region of the LEF-1 gene were performed in Huh7 cells. d ChIP assays were performed in Huh7 cells transfected with FOXG1-siRNA (top), β-catenin-siRNA (middle), and TCF4-siRNA or control siRNA (bottom). e Lysates of 293FT cells expressing FLAG-FOXG1 and myc-TCF4 with or without β-catenin co-transfection were subjected to IP using anti-FOXG1 or anti-TCF4 antibodies, followed by IB with anti-myc or anti-FLAG antibodies. f Lysates of 293FT cells expressed FOXG1 and FLAG-β-catenin with or without myc-TCF4 co-transfection were subjected to IP using anti-FOXG1 or anti-β-catenin antibodies, followed by IB with anti-FLAG or anti-FOXG1 antibody