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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: The Chk1 inhibitor SAR-020106 sensitizes human glioblastoma cells to irradiation, to temozolomide, and to decitabine treatment

Fig. 1

Induction of apoptosis/cell death in LN405 and T98G glioblastoma cell lines. a Dot plot analysis after AnnexinV assay and b histograms presenting apoptotic DNA fragments (sub-G1 fraction) after cell cycle analysis (Nicoletti assay) 4, 24, and 96 h after combined treatment with 0.25 μM SAR and 8 Gy single dose irradiation. One representative experiment is figured in a and b. c, d Joined analyses of apoptosis induction (c AnnexinV/propidium iodide staining; d sub-G1 cell fraction) in both cell lines 96 h after single dose treatment with 0.25 μM SAR, 50 μM TMZ, 0.1 μM 5-aza-dC, and/or 8 Gy IR. Data are weighted means ± medium SEMs from three independent experiments of each cell line. Statistical significance is given compared to untreated, non-irradiated control and indicated by asterisks (*, p ≤ 0.05; ***, p ≤ 0.001), compared to IR alone by hashtags (#, p ≤ 0.05; ##, p ≤ 0.01; ###, p ≤ 0.001). All irradiated groups are significantly different to the control group (p ≤ 0.01) and therefore no further labeling by asterisks is shown

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