Fig. 7

Nestin-positive cells. a-c Potential tumour stem cell population of primary glioblastoma cells P0297 and d, e. Normal neural progenitor cells in murine hippocampal slice cultures both fractionated (7x) treated with 0.1 μM 5-aza-dC, 0.25 μM SAR and 2.2 Gy (total dose 15.4 Gy). a Representative photographs of nestin staining (green) and DAPI nuclear stain (blue). b Double-stained P0297 cells: nestin (green); GFAP (red); DAPI nuclear counterstain (blue). Scale bar of A and B = 20 μm. c Absolute amount of nestin-positive glioblastoma cells. Data are means ± SEM from one experiment with three independent counts of at least 100 cells in total. No significances were detected. d Relative amount of nestin-positive neural progenitor cells within the dentate gyrus normalized to untreated control. Data are means ± SEM, ciphers at the bar bottom indicate the number of animals used. No significances were detected. E Representative photograph of the dentate gyrus (nestin-fluorescent cells, green) and the corresponding analysis by ImageJ (counted cells, black)