Fig. 2

BMP3 inhibits proliferation, migration, invasion and promotes apoptosis in CRC cells. a qRT-PCR and western blot show BMP3 overexpression in HCT116 cells and knockdown efficiency in KM12 cells. b Proliferation of HCT116-BMP3 and KM12-shBMP3 cells was assessed by Cell Counting Kit-8 (CCK-8) assays. c Caspase-3/7 activity in HCT116-BMP3 was measured in cell lysates 24 h after cells spread. d, e Transwell migration (crystal violet staining) and invasion (DAPI staining) assays of HCT116 and KM12 cells were performed after transduction by BMP3-expression or knockdown lentivirus. f Wound healing assays were performed, and cells were photographed every 24 h after scratching. The wound healing percentage represents mean ± SD of at least three experiments at each time point. Statistical results were shown by bar or linear graph. * p < 0.05, ** p < 0.01