Fig. 5

IL-6 increases OCT4 expression through DNMT3b regulation in vitro and in vivo. a Effect of nanaomycin A (a DNMT3b inhibitor) on protein expression of DNMT3b/1, OCT4, and N-cadherin in Hep3B cells. b The effect of nanaomycin A (0, 1, 5, 10, 20 μM) on the migration ability of Hep3B cells. Transwell assay. Bar = 100 um. c Effect of nanaomycin A on IL-6-induced protein expression of DNMT3b/1 and OCT4 in Hep3B cells. d The time course of IL-6/nanaomycin A-treated Hep3B animal models. n = 6 for each group. e Immunohistochemical analysis of the protein expressions of p-STAT3, STAT3, DNMT3b, OCT4, and DNMT1 following PBS, IL-6, and IL-6 plus nanaomycin A treatment (0, 2, 20 μM/mouse/2 days). Bar, 100 μm. f The quantitative data of (a). *P < 0.05, **P < 0.01, ***P < 0.001, student t test