Fig. 5From: Attenuation of hedgehog/GLI signaling by NT1721 extends survival in pancreatic cancerNT1721 inhibited tumor growth and reduced liver metastases in an orthotopic model of pancreatic cancer. Male NSG mice bearing orthotopically growing Panc1 luc+ tumors were treated 12 days after tumor injection with 30 mg/kg NT1721 (three times per week), gemcitabine (100 mg/kg, twice per week) or with the vehicle control. a Bioluminescent signals. The mice were imaged on the indicated days (n = 9). * indicates a significant difference between groups on day 18 (p = 1 × 10− 6). Representative pictures of mice from both groups are shown on the right. b Tumor weight after 5 weeks of treatment with NT1721. c Tumor foci in the liver (n = 8). Tissue slides of livers from mice treated with either NT1721 or gemcitabine for 5 weeks were prepared and the number of tumor foci was quantified as described in the Material and methods section. Representative pictures of whole livers from control and NT1721-treated mice are shown below the graphs. d Comparison of the in vivo efficacy of NT1721 and gemcitabine. The graph shows the percentage of tumor growth in NT1721- and gemcitabine-treated mice compared to the average in control mice (data from 2 independent experiments). e Decreased metastasis formation in liver. The mice (n = 4 per group) were injected with D-luciferin after 5 weeks of treatment with NT1721 or gemcitabine and then euthanized. The livers were immediately harvested and used for bioluminescent imaging. The Mann-Whitney test was used to determine the p values indicated above the graphsBack to article page