Skip to main content

Advertisement

Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Downregulation of MYO1C mediated by cepharanthine inhibits autophagosome-lysosome fusion through blockade of the F-actin network

Fig. 7

Depletion of MYO1C enhances CEP-mediated blockade of autophagosome-lysosome fusion and the accumulation of mitophagosomes. shCon and shMYO1C cells were treated without or with CEP (4 μM) or Rapa (0.25 μM) for 24 h. a Whole-cell lysate was prepared and subjected to immunoprecipitation using anti-LC3 and the associated LAMP1 was determined using immunoblotting. b The fluorescent images showed the colocalization of LC3 (red) and LAMP1 (green). The Pearson’s correlation coefficient (R2) of LC3 and LAMP1 colocalization was from 50 cells of three independent experiments (mean ± SD, n.s, not significant; *P < 0.05; **P < 0.01). c The mitochondrial fraction was prepared and subjected to western blot using antibody against LC3. The ratio of LC3-II/LC3-I was quantified in three independent experiments (mean ± SD, n.s, not significant; **P < 0.01). d The fluorescent images of both shCon and shMYO1C cells transfected with EGFP-LC3 (green) and RFP-Mito (red). Quantification of EGFP-LC3 and RFP-mito colocalization puncta in 50 cells of three independent experiments (mean ± SD, n.s, not significant; **P < 0.01)

Back to article page