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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: Downregulation of MYO1C mediated by cepharanthine inhibits autophagosome-lysosome fusion through blockade of the F-actin network

Fig. 8

Overexpression of MYO1C promotes the interaction/colocalization of MYO1C/F-actin with LC3 and LAMP1. a MDA-MB-231 cells were transfected with control plasmid (Vector) or MYO1C overexpressing plasmid (MYO1C) for 48 h, and the expressing of MYO1C was determined by western blot analysis. Vector or MYO1C cells were treated with CEP (4 μM) and Rapa (0.25 μM) for 24 h. b Whole-cell lysate was prepared and subjected to immunoprecipitation using anti-LC3 and the associated MYO1C and actin were determined using immunoblotting. c The fluorescent images of cells immunostained for MYO1C (blue), F-actin (green) and LC3 (red). d LAMP1 was immunoprecipitated, and the expressions of MYO1C and actin were determined by immunoblotting. The number of LC3 puncta colocalized with MYO1C and F-actin was quantified from 50 cells in three dependent experiments, data was present as mean ± SD (n.s, not significant; *P < 0.05; **P < 0.01) e The fluorescent images of both vector and MYO1C cells immunostained for MYO1C (blue), F-actin (green) and LAMP1 (red). The number of LAMP1 puncta colocalized with MYO1C and F-actin was quantified from 50 cells in three dependent experiments, data was present as mean ± SD (n.s, not significant; *P < 0.05; **P < 0.01)

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