Fig. 2

ERp57 plays a critical role in ccRCC cell proliferation and migration. a qRT-PCR detected ERp57 mRNA level in 6 ccRCC cell lines (Caki-1, A498, 786–0, OSRC-o, SW839 and ACHN). b SW839 cells transfected with shERp57 (shERp57–1 and shERp57–2) or A498 cells transfected with overexpression ERp57 vector (pWPI-ERp57). ERp57 and Cyclin E1 protein level was measured by western blotting. Densitometric analysis from three independent experiments. *P < 0.05, **P < 0.01 vs corresponding control. c and d Cells were prepared as b, cell viability was measured by MTT assay c and colony formation assays d. Right panel shows the number of colony formation. *P < 0.05, **P < 0.01 vs corresponding control. e Cells were treated as (b). Transwell migration assay was used to test migration ability. Right panel shows the number of migration cells. **P < 0.01 vs corresponding control