Fig. 4

NOTCH2 represses EMT in NPC. a Morphological alterations were confirmed by staining for the CNE-2 and 5-8F cell cytoskeletal proteins. b Flow cytometric analysis. shNOTCH2 CNE-2 cells contained an increased proportion of CD133⁺ cells. *P < 0.05 vs. Ctrl cells, one-way ANOVA. c Immunofluorescence showed that suppression of NOTCH2 increased vimentin expression and decreased E-cadherin expression. d Western blots showed that suppression of NOTCH2 increased vimentin expression and decreased E-cadherin expression; NOTCH2 overexpression decreased vimentin expression and increased E-cadherin expression. *P < 0.05, Student’s t-test. Scale bars =50 μm