Fig. 3

C-2-induced autophagy is associated with JNK pathway by alleviating the suppression of Bcl-2 and Bcl-xL on Beclin-1 in bladder cancer cells. a. The phosphorylation of JNK and c-Jun were analyzed by western blotting at indicated concentration or treated with 4 μM of C-2 at indicated time points in BIU87 and EJ cells. b BIU87 and EJ cells were pretreated with 10 μM of SP600125 for 1 h and then treated with 4 μM of C-2 for an additional 24 h. The indicated proteins were detected by western blotting after immunoprecipitation with an antibody for Beclin-1. c The effects of JNK inhibitor SP600125 (10 μM) on 4 μM of C-2-induced LC3-II and p-JNK expression changes of BIU87 and EJ cells