Fig. 5
SP600125 block JNK-SQSTM1/p62-mediated Nrf2 anti-apoptotic pathway. Immunoprecipitation (a) and Western blotting (b) assay showed the effect of SP600125 (10 μM) on the expression changes of proteins in BIU87 cells incubated with 4 μM of C-2 for 6 h. (c) MTT assay demonstrated the effect of SP600125 (10 μM) on the cell viability of BIU87 and EJ treated by 4 μM of C-2 for 6 h. **P < 0.02 vs C-2 treatment group. (d) Western blotting assay showed the effect of JNK siRNA (20 nM) on expression of p62 and p-Nrf2 proteins in BIU87 cells. (e) MTT assay detected the effect of siRNA targeting to JNK on the survival rate of BIU87 cells incubated with 4 μM of C-2 for 6 h, **P < 0.02 vs C-2 treatment group