Fig. 7

HOTAIR mediates metastasis and tumorigenesis of CRC cells in vivo. a The lung metastasis degree of transfected SW480 cells was assessed (up panel). HE staining was presented (down panel). b Lung metastasis model was established by SW620 cells transfected with shHOTAIR. The lung metastasis was inhibited by adding AMG-208. c The liver metastasis was established with the cells transfected with LV-HOTAIR or LV-NC. d CRC liver metastasis model were built with transfected SW620 cells (up panel), and HE staining was presented (down panel). e GFP signals of xenograft tumors were obtained with NS and AMG-208. ST6GAL1 and Ki67 levels were measured by IHC staining. Data were means ± SD of three independent assays (*P < 0.05)