Fig. 3
IGF2BP2 promotes pancreatic cancer proliferation of pancreatic cancer in vitro. a-b GSEA analysis comparing IGF2BP2 high-expression group (red) to the IGF2BP2 low-expression group (blue) of pancreatic cancer tissues. c IGF2BP2 protein levels after transfection analyzed by western blot assay. d and e CCK-8 assay of cell viability in BxPC-3 and Panc-1 cell lines after transfection. Cell viability was determined at 24, 48, 72 and 96 h. f and g Colony formation assay of cell viability in BxPC-3 and Panc-1 cell lines after transfection. h Flow cytometry assay of apoptosis of BxPC-3 and Panc-1 cells after transfection. i and j Flow cytometry assays of cell cycle distribution of BxPC-3 and Panc-1 cells after transfection. The percentage of cells in each cell cycle phase is shown in the inset of each panel. GAPDH served as a loading control. Data are represented as the mean ± SD of at least three independent measurements. *P < 0.05, ** P < 0.01, and ***P < 0.001. P < 0.05 was considered statistically significant