Fig. 6

PI3K-C2β downregulation enhances docetaxel-induced multi-nucleation. (a-c) PC3 and the indicated stable cell lines were plated on coverslips (a, b). Alternatively, PC3 cells plated on coverslips were transfected with the indicated siRNAs or transfection reagent alone (c). The day after plating (a, b) or after transfection (c), cells were treated with 0.5 nM docetaxel (or corresponding amount of DMSO) and incubated for further 72 h. Cells were then fixed and stained with anti α-tubulin (green) and DAPI. Representative images of the indicated stable cells treated with 0.5 nM docetaxel are shown in (a). Graphs in (b, c) indicate the number of docetaxel-treated cells containing n = 1, n = 2 or n ≥ 3 (multi-nucleated) nuclei, expressed as percentage of total number of cells. Results from corresponding cells treated with vehicle alone are presented in Additional file 11: Figure S10a, b. Data in (b) are means ± s.e.m. of n = 6 (PC3 and sh scrambled), n = 4 [sh PI3K-C2β (3)] and n = 3 [sh PI3K-C2β (4)] independent experiments. The total numbers of cells analysed in these experiments were as follows: PC3 DMSO: 6254; PC3 docetaxel: 4250; sh scrambled (4) DMSO: 5942; sh scrambled (4) docetaxel: 3778; sh PI3K-C2β (3) DMSO: 3994; sh PI3K-C2β (3) docetaxel: 2773; sh PI3K-C2β (4) DMSO: 2372; sh PI3K-C2β (3) docetaxel: 2013. ***p < 0.001 vs corresponding PC3; ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0001 vs corresponding sh scrambled (4) (one tailed, unpaired t-Test with Welch’s correction). Data in (c) are means ± s.e.m. of n = 3–5 independent experiments. The total numbers of cells analysed in these experiments were as follows: oligo DMSO: 3553; oligo docetaxel: 2266; si control DMSO: 3986; si control docetaxel: 2746; si PI3K-C2β (1) DMSO: 3571; si PI3K-C2β (1) docetaxel: 2734; si PI3K-C2β (2) DMSO: 2239; si PI3K-C2β (2) docetaxel: 2136. *p < 0.05, **p < 0.01 vs corresponding oligo; ^$p < 0.05 vs corresponding si control (one tailed, unpaired t-Test with Welch’s correction)