Fig. 2

ALOX12–12-HETE was activated during liver IRI in NAFLD. a, b Immunoblot analysis of ALOX12 in CD and HFD feeding mice after IRI. Protein levels were normalized to GAPDH and analyzed. (n = 3 per group) c, d Immunoblot analysis of ALOX12 in AML12 cells stimulation with PA followed by HR. Protein levels were normalized to GAPDH and analyzed. (n = 3 per group) e. qPCR analysis of ALOX12 in CD and HFD feeding mice after IRI. (n = 4–5 per group) f. qPCR analysis of ALOX12 in AML12 cells stimulation with PA followed by HR. (n = 4–5 per group) g, h 12-HETE in serum and in cell supernatant were measured. (n = 4–5 per group) i-l Representative immunofluorescence staining of ALOX12. Scale bars, 100 μm. (n = 3 per group) Data are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired Student’s t- test