Fig. 7

GPR31 mediates the IRI induced HCC recurrence in NAFLD. a-c qPCR analysis of FFAR1, FFAR2, FFAR3, FFAR4, GPR31and GPR75 in Hepa1–6, bel-7402 and Huh7 cells stimulated with 12-HETE. (n = 4–5 per group). d-f qPCR and immunoblot analysis of GPR31 in bel-7402 and Huh7 cells with Si-GPR31 or Si-Con. Protein levels were normalized to GAPDH and analyzed. (n = 3–4 per group) g, h. Immunoblot analysis of PI3K, AKT, NFκB, E-cadherin, N-cadherin, Vimentin, Snail, Slug, MMP2, MMP7, MMP9 and MMP13 in bel-7402 and Huh7 cells with Si-GPR31 and 12-HETE stimulation. i, j. Representative images and HRA of HCC recurrence in HFD mice suffered IRI and inoculated with GPR31^−/− Hepa1–6 cells. Scale bars, 1 cm. (n = 3–4 per group) f Representative H&E staining of liver sections of HCC recurrence in HFD mice suffered IRI and inoculated with GPR31^−/− Hepa1–6 cells. Scale bars, 200 μm. Data are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired Student’s t- test