Fig. 5

NF-YAx attenuates HEK-293, SH-SY5Y and ST14A colony formation. a Representative Micrographs (bar = 100 μm) plus histograms, demonstrating Necrostatin-1 (Nec-1) (100 μM) but not z-VAD-fmk (10 μM) inhibition of NF-YAx-induced SH-SY5Y and HEK-293 cell-death. Histogram data are displayed as mean (± SD) percent adherent/alive and suspension/dead cells, in three independent assays performed in duplicate (* = significant). b Representative colony formation assays plus histograms, demonstrating inhibition of HEK293, SH-SY5Y and ST14A colony-formation by NF-YAx and NF-YAdn compared to NF-YAl, NF-YAs and pcDNA expression vectors. Histogram data are displayed as mean (± SD) percentage difference to pcDNA-transfected controls, in three independent assays performed in triplicate (* = significant)