Fig. 3
From: HOXC-AS1-MYC regulatory loop contributes to the growth and metastasis in gastric cancer
HOXC-AS1 was transcriptionally upregulated by c-MYC in GC. (a) Three online tools including UCSC, JASPAR and PROMO predicted that c-MYC might potentially regulate HOXC-AS1 transcription. (b, e) The expression of MYC and HOXC-AS1 in GC cells with MYC downregulation or overexpression was assayed by qRT-PCR. (f, g) ChIP and luciferase reporter assays revealed that HOXC-AS1 was positively regulated by c-MYC at transcriptional level. (h, i) The predicted binding of c-MYC on HOXC-AS1 promoter region was simulated here. (j) Luciferase reporter assay confirmed the pricise binding of c-MYC to HOXC-AS1 promoter at site from − 1954 to − 1959. * P < 0.05, ** P < 0.01