Fig. 5

HOXC-AS1 promoted BRG1 interaction with β-catenin to enhance MYC transcription. (a) qRT-PCR result of the expression of MYC in BGC-823 and AGS cells in response to HOXC-AS1 silence or not, or HOXC-AS1 silence plus miR-590-3p inhibition. (b) The effect of HOXC-AS1 on MYC transcription was assessed by luciferase reporter assay. (c) The binding of HOXC-AS1 to MYC promoter was examined by DNA pull down assay. (d) TOP/FOP flash assay was conducted to estimate the impact of HOXC-AS1 on Wnt/β-catenin activation. (e, f) The influence of HOXC-AS1 on CTNNB1 expression and β-catenin nuclear translocation was determined by qRT-PCR and western blot, as appropriate. (g) The interaction between HOXC-AS1 and BRG1 in GC cells was testified by RIP and RNA pull down assays. (h) The interaction between BRG1 and β-catenin in GC cells with or without HOXC-AS1 knockdown was evaluated by CoIP assay. ** P < 0.01