Fig. 6
From: HOXC-AS1-MYC regulatory loop contributes to the growth and metastasis in gastric cancer
Silencing HOXC-AS1 confined GC tumorigenesis and metastasis in vivo. (a) Representative images and tumor volume of AGS cells transfected with shCtrl, shHOXC-AS1, or shHOXC-AS1 + pcDNA3.1/MYC. (b) Mean weight of these tumors. (c) qRT-PCR result of the expression of HOXC-AS1 and MYC in above tumors. (d) The expression of HOXC-AS1, MYC, Ki67, E-cadherin and N-cadherin in those tumors was tested by ISH or IHC staining, as needed. (e) The level of EMT-related proteins was determined by western blot. (f, g) HE staining of livers and lung obtained from in vivo metastatic experiments and quantitation of the metastatic nodules in these livers. ** P < 0.01