Fig. 7
From: HOXC-AS1-MYC regulatory loop contributes to the growth and metastasis in gastric cancer
MYC upregulation reversed the suppression of HOXC-AS1 inhibition on the biological processes of GC cells. (a) qRT-PCR and western blot analyses were performed to assay the expression of MYC at both mRNA and protein levels in AGS cells transfected with shCtrl, shHOXC-AS1#1 or shHOXC-AS1#1 together with pcDNA3.1 plasmid containing MYC. (b-e) The viability, proliferation, apoptosis and motility in above AGS cells were assessed respectively by CCK-8, EdU, TUNEL and transwell assays. (f) The level of EMT-associated proteins in indicated AGS cells was determined by western blot. * P < 0.05, ** P < 0.01