Fig. 3

CircRHOBTB3 inhibited proliferation of GC cells in vitro. a, b cck8 assay of AGS transfected with control lentivirus (NC) or LV-SH-circRHOBTB3 (SH-circRHOBTB3) and MKN45 transfected with lentivirus vector or over-expressed circRHOBTB3 letivirus. c, d Colony formation assay and Edu assay were conducted to evaluated cell proliferative ability in stable silenced circRHOBTB3 AGS cells or over-expressed circRHOBTB3 MKN45 cells. e, f Representative images of cell cycle in AGS cells after silencing circRHOBTB3 or MKN45 cells after elevating circRHOBTB3 were analyzed by flow cytometry. The data was expressed as the mean ± SD and reproduced in three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001