Fig. 6

hBMSCs-derived exosomal miR-205 suppresses RHPN2 expression in LNCaP cells. a co-culture pattern of hBMSCs or hBMSC-derived exosomes with LNCaP cells; b the internalization of exosomes by prostate cancer cells observed under a confocal microscope (× 400); c-d RHPN2 expression after co-culture of hBMSCs with LNCaP cells determined using RT-qPCR and western blot analysis; e-f RHPN2 expression after co-culture of exosomes from hBMSCs with LNCaP cells determined using RT-qPCR and western blot analysis; g the binding of hBMSCs-derived exosomes with Cy3-labeled red fluorescence to LNCaP cells observed under a confocal microscope; exosomes carrying Cy3-labeled miR-205 were red, nuclei stained with DAPI were blue; cells labeled with phalloidin were stained green. Scale bar = 20 μm; *, p < 0.05. Measurement data were expressed as mean ± standard deviation; comparisons between two groups were analyzed using unpaired t-test; the experiment was repeated three times independently