Fig. 2

Effects of Olaparib treatment on apoptosis/autophagy induction in OC cell lines. UWB, UWB-BRCA and SKOV3 cells were treated for 144 h with Olaparib. a) The percentages of early apoptotic and late apoptotic cells were obtained by flow cytometry quadrant analysis with annexin A5 FITC/7-AAD double staining, and expressed as histograms. b) The expression of the apoptosis related proteins phAKT (Ser473), AKT, cleaved Caspase 3 and cleaved PARP1 was determined by Western blot analysis. Tubulin expression was used as internal control. The images are representative of at least two independent experiments. CF, cleaved form of PARP1. c) The expression of the autophagy related proteins LC3 and P62 was assessed by Western blot analysis. d) UWB and UWB-BRCA cells were treated for 144 h with Olaparib (5 μM), Chloroquine (CQ, 10 μM) or a combination of them, and the expression of LC3 and P62 was assessed. The intensity of the LC3-II and P62 bands was evaluated by densitometric analysis, normalized with LC3-I and Tubulin, respectively, and reported in graph. Error bars represent standard deviations. *, p < 0.05, **, p < 0.005 vs. control (DMSO)