Fig. 8
From: MiR-200c sensitizes Olaparib-resistant ovarian cancer cells by targeting Neuropilin 1
Effect of miR-200c overexpression on Olaparib-induced apoptosis. SKOV3 cells were stably transfected with a plasmid carrying the precursor of miR-200c (miR-200c) and its corresponding vector control (CTRL), then treated for 144 h with Olaparib. a) The percentages of early apoptotic and late apoptotic cells were obtained by flow cytometry quadrant analysis with annexin A5 FITC/7-AAD double staining, and expressed as histograms. b) The expression of the apoptosis related proteins phAKT (Ser473), AKT, cleaved Caspase 3 and cleaved PARP1 was determined by Western blot analysis. Tubulin expression was used as internal control. The images are representative of at least two independent experiments. CF, cleaved form of PARP1