Fig. 4

FoxP3 as the transcription repressor of DR4 and DR5. a. Predictions of shared transcription factors in DR4 and DR5 promoters (Version 8.3 of TRANSFAC. b. Western blot analysis of the change in the overall levels of FoxP3 and the change of FoxP3 in nucleus and cytoplasm after drug treatment. c. Immunohistochemical analysis of FoxP3 expression in 50 cases of ESCC specimens and 33 cases of adjacent specimens. Scale bar, 200 μm (above); Scale bar, 50 μm (below). d. Western blot analysis of the expression levels of FoxP3, DR4 and DR5 proteins. YES-2 and KYSE-150 cells were transfected with FoxP3 expression plasmid or vector (pcDNA3.1) after treatment with various concentrations of CPP alone for 24 h. e-f. qRT-PCR analysis of the expression levels of DR4 and DR5 mRNA. YES-2 and KYSE-150 cells were transfected with FoxP3 expression plasmid or vector (pcDNA3.1) after treatment with various concentrations of CPP alone for 24 h